Biologists have often wondered can penguins be gay? One of the most peculiar things about penguins is that it’s not easy to tell at the first look if the penguin is a male or a female. In most cases, it is only possible through a DNA test to find out if a penguin is a male or a female. This is one reason why finding male penguin pairs in nature can be a hit or miss venture. But in captivity and penguins often pair up with another penguin of the same sex. Even when introduced to female penguins, these penguins will not leave their same-sex partner.